Many peptide users often end up disappointed with their peptides, not because they received the wrong peptide, but they messed up because they did not know how to handle and store them properly. This is something you must watch out for, if you want to avoid getting poor results or contaminating your peptides all the time. Here, we take a brief look at what is expected of you, as far as handling synthesized peptides is concerned.
Shipping done at room temperature
To begin with, it is important to understand that in whatever form you order your peptides, shipping will be done at room temperature, but this is never the recommended temperature for storage.
Storage guidelines for synthesized peptides
For long-term storage, the ideal temperature recommended for lyophilized peptides is -200C, or at -800C, but with a desiccant in sealed containers to reduce the chances of the peptides getting impacted. Under such conditions, it becomes possible to store peptides for years, without them being affected by bacterial degradation, formation of secondary structures, or being affected by oxidation.
Opening the package
Before opening and weighing peptides, it is highly recommended that you equilibrate them to room temperature in a desiccator. Failure to do so may cause condensation to form on them, the moment the bottle is opened, and this will likely affect the stability of the peptides.
While weighing the peptides, weigh the required quantity rapidly, and take back the remaining portion back to storage, under a temperature of below -200C. Be advised that sequences with asparagine, glutamine, N-terminal glutamic acid, methionine, cysteine, and tryptophan, will generally have a shorter lifespan than the other peptides.
After you are done measuring and weighing the peptides, centrifuge the vial at 12,000 x g for about 20 seconds, to ensure that they are ready for reconstitution.